Crest CARVII™Applications and Images

Multichannel confocal imaging

The Crest CARV II™ imager is suitable for both live cell and fixed cell confocal imaging using a wide variety of fluorophores.

Stack projection of HeLa cells with Qdot® conjugates. Nucleus – Qdot® 655; Golgi – Qdot® 585; Microtubules – Qdot® 525. Quantum Dot Corporation, Hayward, CA.

Stack projection of mouse intestine section labeled with Alexa Fluor® 350 WGA, Alexa Fluor® 568 phalloidin, and SYTOX® Green. Molecular Probes/Invitrogen, Eugene, OR.

3D reconstruction of imaged skin sample labled using pan-neuronal marker, protein gene product 9.5 localized with Cy™3 and basement membrane marker, type IV collagen with CY2. Endothelial cells are stained using Cy™5-Ulex europaeus agglutinin type I. Dr. William R. Kennedy and Gwen Wendelshafer-Crabb, University of Minnesota, Minneapolis, MN.