Neuronal Monitoring
- Chicken cerebral cortex
- To observe elevator activity (interkinetic nuclear movement by time-lapse imaging) in mouse neurons
- FP: GFP
- Time lapse interval 30 min., up to 40 hours, 5 fields, Z thickness 40µ (11 slices at 4µ interval)
- EX: 488nm
- Objective lens: 20X 0il
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Neuronal Monitoring
- Brain slice of mouse fetus
- To observe particle movement
- FP: GFP, Strawberry (position marker)
- EX: 488nm 10%, 561nm 12%
- EMCCD exposure and gain: 488nm 300ms, 100%, 561nm 200ms, 100%
- Objective lens: 60X oil
- Conditions: time lapse, 1 min. interval up to 5 hours, 1 field
- Z thickness 30µ (61 slices at 0.5µ interval)
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